Overview

  • Product nameAnti-CHD3 antibody [EPNCIR110A]
    See all CHD3 primary antibodies
  • Description
    Rabbit monoclonal [EPNCIR110A] to CHD3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse CHD3 aa 1-100. The exact sequence is proprietary.
    Database link: B1AR17

  • Positive control
    • K562, HeLa, C6, and PC12 cell lysates, Human breast and Human ovary tissues; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager.  View antibodies from NCI Center for Cancer Research Collaboration 

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 226 antibody
      • 2600010P09Rik antibody
      • AF020312 antibody
      • ATP dependent helicase CHD3 antibody
      • ATP-dependent helicase Chd3 antibody
      • CHD-3 antibody
      • Chd3 antibody
      • CHD3_HUMAN antibody
      • Chd7 antibody
      • Chromodomain-helicase-DNA-binding protein 3 antibody
      • hZFH antibody
      • MGC40857 antibody
      • Mi 2 autoantigen 240 kDa protein antibody
      • Mi 2a antibody
      • Mi-2 autoantigen 240 kDa protein antibody
      • Mi2 ALPHA antibody
      • Mi2-alpha antibody
      • Prp7 antibody
      • Prp9 1 antibody
      • ZFH antibody
      • Zinc finger helicase antibody
      • zinc-finger helicase (Snf2-like) antibody
      see all

    Anti-CHD3 antibody [EPNCIR110A] images



    • Predicted band size : 227 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
      Lane 3: Raji cell lysate (20 µg)
      Lane 4: Y79 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 227 kDa
      Observed band size : 260 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.
    • Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.
    • Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.

    References for Anti-CHD3 antibody [EPNCIR110A] (ab109195)

    ab109195 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"