Overview

  • Product name
  • Description
    Mouse monoclonal to CHD4
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ETEPKGAADV EKVEEKSAID LTPIVVEDKE EKKEEEEKKE VMLQNGETPK DLNDEKQKKN IKQRFMFNIA DGGFTELHSL WQNEERAATV TKKTYEIWH, corresponding to amino acids 1632-1731 of Human CHD4

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab54603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 218 kDa.
IHC-P Use a concentration of 3 µg/ml.

Target

  • Function
    Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

Anti-CHD4 antibody images

  • CHD4 antibody (ab54603) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human transitional cell carcinoma.


  • Predicted band size : 218 kDa
    CHD4 antibody (ab54603) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab54603 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54603, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG2a [ICIGG2A], (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-CHD4 antibody (ab54603)

This product has been referenced in:
  • Trakarnsanga K  et al. Induction of adult levels of ß-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL. Haematologica 99:1677-85 (2014). WB . Read more (PubMed: 25107887) »
  • Sparmann A  et al. The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. EMBO J 32:1598-612 (2013). IHC-P . Read more (PubMed: 23624931) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - other (U2OS cells)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
10 µg
Specification
U2OS cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 11 2015

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - nuclear (U2OS)
Specification
U2OS
Treatment
500nM Cisplatin
Total protein in input
500 µg
Username

Abcam user community

Verified customer

Submitted Nov 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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