Overview

  • Product nameAnti-CHD4 antibody
    See all CHD4 primary antibodies
  • Description
    Rabbit polyclonal to CHD4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.

  • Positive control
    • This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) Nuclear whole cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa).

Target

  • FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

Anti-CHD4 antibody images

  • Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 218 kDa
    Observed band size : 218 kDa
    Additional bands at : 120 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-CHD4 antibody (ab85620)

ab85620 has not yet been referenced specifically in any publications.

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