Anti-CHD4 antibody (ab85620)

Overview

• Product name
  Anti-CHD4 antibody
  See all CHD4 primary antibodies
• Description
  Rabbit polyclonal to CHD4
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Horse, Chicken
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.

  Read Abcam's proprietary immunogen policy

• Positive control
  • This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) nuclear lysate.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Immune System Diseases
  • Autoimmune

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Mi-2

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
• Sequence similarities
  Belongs to the SNF2/RAD54 helicase family.
  Contains 2 chromo domains.
  Contains 1 helicase ATP-binding domain.
  Contains 1 helicase C-terminal domain.
  Contains 2 PHD-type zinc fingers.
• Post-translational
  modifications
  Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localization
  Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.

• Target information above from: UniProt accession Q14839 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 1108 Human
  • Entrez Gene: 107932 Mouse
  • Entrez Gene: 117535 Rat
  • Omim: 603277 Human
  • SwissProt: Q14839 Human
  • SwissProt: Q6PDQ2 Mouse
  • Unigene: 162233 Human
  • Unigene: 333388 Mouse
• Alternative names
  • ATP dependent helicase CHD4 antibody
  • ATP-dependent helicase CHD4 antibody
  • CHD 4 antibody

  • CHD-4 antibody
  • CHD4 antibody
  • CHD4_HUMAN antibody
  • Chromodomain helicase DNA binding protein 4 antibody
  • Chromodomain-helicase-DNA-binding protein 4 antibody
  • Mi 2 autoantigen 218 kDa protein antibody
  • Mi 2b antibody
  • Mi-2 autoantigen 218 kDa protein antibody
  • Mi2 beta antibody
  • Mi2-beta antibody

  see all

Protocols

• Western blot protocols