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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa).
Function Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Sequence similarities Belongs to the SNF2/RAD54 helicase family. Contains 2 chromo domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 PHD-type zinc fingers.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
Information by UniProt
ATP dependent helicase CHD4 antibody
ATP-dependent helicase CHD4 antibody
CHD 4 antibody
Anti-CHD4 antibody images
Western blot - CHD4 antibody (ab85620)
Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 218 kDa Observed band size : 218 kDa Additional bands at : 120 kDa,31 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
References for Anti-CHD4 antibody (ab85620)
has not yet been referenced specifically in any publications.
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