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Rabbit polyclonal to CHD4
Predicted to work with:
Mouse, Rat, Horse, Chicken
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.
This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) nuclear lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa).
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 PHD-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
Information by UniProt
ATP dependent helicase CHD4 antibody
ATP-dependent helicase CHD4 antibody
CHD 4 antibody
Western blot - CHD4 antibody (ab85620)
Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
120 kDa,31 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"