- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CHD4 antibody
See all CHD4 primary antibodies -
DescriptionRabbit polyclonal to CHD4
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.
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Positive control
- This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) nuclear lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab85620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa). |
Target
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FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
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Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
- Information by UniProt
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Database links
- Entrez Gene: 1108 Human
- Entrez Gene: 107932 Mouse
- Entrez Gene: 117535 Rat
- Omim: 603277 Human
- SwissProt: Q14839 Human
- SwissProt: Q6PDQ2 Mouse
- Unigene: 162233 Human
- Unigene: 333388 Mouse
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Alternative names
- ATP dependent helicase CHD4 antibody
- ATP-dependent helicase CHD4 antibody
- CHD 4 antibody
see all
Images
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Western blot - CHD4 antibody (ab85620)Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 218 kDa
Observed band size: 218 kDa
Additional bands at: 120 kDa, 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Abcam recommends loading unheated lysates and using 20% Ethanol and 0.04% SDS in the transfer buffer to obtain a positive signal for this antibody. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above
Protocols
References
ab85620 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"