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Synthetic peptide corresponding to a region between residues 25 and 75 of human CHD4 (NP_001264.2)
Our Abpromise guarantee covers the use of ab72418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use a concentration of 2 - 20 µg/ml. PubMed: 21670149|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23911933|