• Product nameAnti-CHD4 antibody - ChIP Grade
    See all CHD4 primary antibodies
  • Description
    Rabbit polyclonal to CHD4 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, IHC-P, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 25 and 75 of human CHD4 (NP_001264.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.



Our Abpromise guarantee covers the use of ab72418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use a concentration of 2 - 20 µg/ml. PubMed: 21670149
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 23911933


  • FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

Anti-CHD4 antibody - ChIP Grade images

  • All lanes : Anti-CHD4 antibody - ChIP Grade (ab72418) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 218 kDa
    Observed band size : 280 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Detection of CHD4 by Western Blot of Immunprecipitate.
    ab72418 at 0.1µg/ml staining CHD4 in HeLa whole cell lysate immunoprecipitated using ab72418 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.
  • ab72418 (1µg/ml) staining CHD4 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining within the colonic mucosa.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-CHD4 antibody - ChIP Grade (ab72418)

This product has been referenced in:
  • Kim JY  et al. Specificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor. Mol Cell 56:738-48 (2014). Read more (PubMed: 25453762) »
  • Yüce Ö & West SC Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response. Mol Cell Biol 33:406-17 (2013). WB . Read more (PubMed: 23149945) »

See all 8 Publications for this product

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