Overview

  • Product name
  • Description
    Rabbit polyclonal to CHD6
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 2665- 2715 of Human CHD6 (NP_115597.3).

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114095 was affinity purified using an epitope specific to CHD6 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 305 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Probable transcription regulator.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase CHD6 antibody
    • ATP-dependent helicase CHD6 antibody
    • CHD-6 antibody
    • CHD5 antibody
    • CHD6 antibody
    • CHD6_HUMAN antibody
    • chromodomain helicase DNA binding protein 6 antibody
    • Chromodomain-helicase-DNA-binding protein 6 antibody
    • helicase C-terminal domain- and SNF2 N-terminal domain-containing protein antibody
    • KIAA1335 antibody
    • Radiation induced gene B protein antibody
    • Radiation-induced gene B protein antibody
    • RIGB antibody
    • RP4-620E11.2 antibody
    see all

Images

  • All lanes : Anti-CHD6 antibody (ab114095) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 305 kDa


    Exposure time : 30 seconds
  • ab114095, at 1 µg/ml, staining CHD6 in HeLa whole cell lysate immunoprecipitated using ab114095 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab114095 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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