The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355
1/100 - 1/500.
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Use at an assay dependent concentration. PubMed: 23285124
FunctionDNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded mouse teratoma by Immunohistochemistry using DAB staining.
References for Anti-CHD8 antibody (ab84527)
This product has been referenced in:
Tahara T et al. Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology146:530-538.e5 (2014).
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