Overview

  • Product name
  • Description
    Rabbit polyclonal to Chemerin
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-163 of Human Chemerin (NP_002880.1).

  • Positive control
    • Mouse testis lysate; Chemerin transfected 293T cell line lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab103153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificity
    Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HP10433 antibody
    • RAR responsive protein TIG2 antibody
    • RAR-responsive protein TIG2 antibody
    • RARR2_HUMAN antibody
    • RARRES2 antibody
    • Retinoic acid receptor responder (tazarotene induced) 2 antibody
    • Retinoic acid receptor responder 2 antibody
    • Retinoic acid receptor responder protein 2 antibody
    • Tazarotene induced gene 2 protein antibody
    • Tazarotene-induced gene 2 protein antibody
    • TIG2 antibody
    see all

Images

  • ICC/IF image of ab103153 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103153 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Chemerin antibody (ab103153) at 1/500 dilution + Mouse testis lysate at 50 µg

    Predicted band size : 19 kDa
  • All lanes : Anti-Chemerin antibody (ab103153) at 1/500 dilution

    Lane 1 : Chemerin transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 19 kDa

References

ab103153 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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