For the best experience on the Abcam website please upgrade to a modern browser such as
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
Use at an assay dependent concentration.
Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon.
Information by UniProt
RAR responsive protein TIG2 antibody
RAR-responsive protein TIG2 antibody
Anti-Chemerin antibody images
Western blot - Chemerin antibody (ab72965)
All lanes :
Anti-Chemerin antibody (ab72965) at 1/500 dilution
Lane 1 :
Chemerin transfected 293T cell lysate
Lane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size :
Observed band size :
15 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Chemerin antibody (ab72965)
ICC/IF image of ab72965 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Chemerin antibody (ab72965)
This product has been referenced in:
et al. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration. Oncotarget 7:83554-83569 (2016).
Read more (PubMed: 27907906) »
et al. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes 58:2731-40 (2009).
Read more (PubMed: 19720798) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"