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A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities
Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.
Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.
|luminol reagent.||1 x 20ml|
|reaction buffer.||2 x 20ml|
Our Abpromise guarantee covers the use of ab5801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.