Chemiluminescent HRP Substrate (membrane) (ab5801)


  • Product nameChemiluminescent HRP Substrate (membrane)
  • Product overview

    A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.


    The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities

  • Notes

    Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.

    Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.

  • Tested applicationsSuitable for: WBmore details



    Our Abpromise guarantee covers the use of ab5801 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
  • Application notesWB: Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.

    Not tested in other applications.
    Optimal incubation time should be determined by the end user.
  • References for Chemiluminescent HRP Substrate (membrane) (ab5801)

    This product has been referenced in:
    • Pacini S  et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). Read more (PubMed: 20360837) »

    See 1 Publication for this product

    Product Wall

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