Anti-Chemokine Receptor D6 antibody (ab1656)

Overview

  • Product nameAnti-Chemokine Receptor D6 antibody
    See all Chemokine Receptor D6 primary antibodies
  • Description
    Goat polyclonal to Chemokine Receptor D6
  • SpecificityPeptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
  • Tested applicationsSuitable for: Flow Cyt, ICC, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    SSSIYDYDYLDDMTILVCRKDEVLSFGRC

    , corresponding to amino acids 18-45 of Mouse Chemokine Receptor D6

  • Positive control
    • Mouse spleen cells or lung.
  • General notes


    Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesReceptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. 1-3µl/106cells/50-100µl.

ICC 1/500.
IHC-P 1/250.
ELISA 1/100000.

Target

  • FunctionReceptor for C-C type chemokines including SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3.
  • Tissue specificityExpressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node. Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C chemokine receptor D6 antibody
    • C-C chemokine receptor D6 antibody
    • CC chemokine binding receptor JAB61 antibody
    • CC chemokine receptor D6 antibody
    • CCBP 2 antibody
    • CCBP2 antibody
    • CCBP2_HUMAN antibody
    • CCR 10 antibody
    • CCR 9 antibody
    • CCR10 antibody
    • CCR9 antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C) receptor 9 antibody
    • Chemokine binding protein 2 antibody
    • Chemokine binding protein D6 antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 9 antibody
    • Chemokine CC motif receptor 9 antibody
    • Chemokine CC receptor 9 antibody
    • Chemokine receptor 9 antibody
    • Chemokine receptor CCR 10 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR-10 antibody
    • Chemokine receptor CCR-9 antibody
    • Chemokine receptor CCR10 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine receptor D6 antibody
    • Chemokine-binding protein 2 antibody
    • Chemokine-binding protein D6 antibody
    • CMKBR 9 antibody
    • CMKBR9 antibody
    • D6 antibody
    • hD6 antibody
    • MGC126678 antibody
    • MGC138250 antibody
    see all

Anti-Chemokine Receptor D6 antibody images

  • ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse lung section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-Chemokine Receptor D6 antibody (ab1656)

This product has been referenced in:
  • Hajrasouliha AR  et al. Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection. Mol Vis 19:2517-25 (2013). Flow Cyt ; Mouse . Read more (PubMed: 24357920) »
  • Kin NW  et al. DNA Microarray Gene Expression Profile of Marginal Zone versus Follicular B Cells and Idiotype Positive Marginal Zone B Cells before and after Immunization with Streptococcus pneumoniae. J Immunol 180:6663-74 (2008). WB, Flow Cyt ; Mouse . Read more (PubMed: 18453586) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 27 2012

Thank you for submitting an Abreview of ab1656. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested species, we would like to follow up on this to see if we c...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Thank you for calling Abcam yesterday.
I have talked to the lab and I am sorry but we do not have an immunohistochemistry image showing the use of the blocking peptide with the antibody.
As we talked about yesterday as well, please find below...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from blood)
Specification cells from blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy on lymphocytes
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: from whole blood
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Formaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Nov 08 2010

Thank you for your email and I'm sorry to hear that you are still experiencing difficulty with ab1656. You should definitely see positive staining with the spleen and lung tissue and so it seems as if the antibody is damaged in some way. I can have a r...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1656. Our source for this antibody was able to provide me with the IHC-P protocol below, which I hope will be helpful. Also, mouse spleen or lung tissue is rec...

Read More

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1656. Our source for this antibody was able to provide me with the IHC-P protocol below, which I hope will be helpful. Also, mouse spleen or lung tissue is rec...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"