Overview

  • Product nameAnti-CHERP antibody
    See all CHERP primary antibodies
  • Description
    Rabbit polyclonal to CHERP
  • Tested applicationsSuitable for: IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptides, post synthetically modified to achieve desired antigenicty.

  • Positive control
    • CHERP mRNA is expressed ubiquitously in various tissues including lung, brain, heart, pancreas, kidney and skeletal muscle.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferAntibody stabilization buffer.
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAffinity purified against immobilised antigen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/200.
WB 1/500. Predicted molecular weight: 106 kDa.
ICC 1/200.

Target

  • RelevanceThe Calcium homoeostasis endoplasmic reticulum protein (CHERP) gene has been recently identified and localized on chromosome 19p13.1. CHERP has high homology to an SR related CTD protein, which is known to interact with the largest subunit of RNA polymerase II. CHERP is an integral endoplasmic reticulum membrane protein is involved in calcium mobilization induced by thrombin. Confocal microscopy revealed that CHERP is associated with Ins(1,4,5)P3 receptor throughout the cytoplasm and perinuclear region in Jurkat T lymphocyte. CHERP Anti sense treatment induced a decrease in CHERP, which caused an impaired increase in free cytoplasmic calcium but calcium influx remains unaffected, with some deficient in endoplasmic reticulum calcium stores. In the CHERP depleted Jurkat T lymphocytes the calcium dependent translocation of nuclear factor of activated T cells (NFAT) from cytoplasm to nucleus was also suppressed with significant suppression of cell.
  • Cellular localizationCytoplasm. Cytoplasm; perinuclear region. Endoplasmic reticulum. Note: Distributed throughout the cytoplasm and also localizes to the perinuclear region of both human erythroleukemia (HEL) cells and Jurkat cells. Colocalizes with ITPR1.
  • Database links
  • Alternative names
    • Calcium homeostasis endoplasmic reticulum protein antibody
    • DAN16 antibody
    • DAN26 antibody
    • ERPROT 213 21 antibody
    • ERPROT21321 antibody
    • Protein with polyglutamine repeat antibody
    • SCAF6 antibody
    • SR related CTD associated factor 6 antibody
    • SRA1 antibody
    see all

Anti-CHERP antibody images

  • Immunofluorescence analysis of HEK293 cells, staining CHERP with ab15951.
    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA. Cells were incubated with primary antibody at 4°C overnight. A fluorescent-conjugated anti-rabbit IgG was used as a secondary antibody.

References for Anti-CHERP antibody (ab15951)

This product has been referenced in:
  • Lin-Moshier Y  et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem 288:355-67 (2013). WB, ICC/IF . Read more (PubMed: 23148228) »
  • Ryan T  et al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem 286:17060-8 (2011). WB ; Human . Read more (PubMed: 21454501) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. The immunogen, a peptide (17mer), was synthesized, and the sequence is taken from within the C-terminal region spanning between aa 875-916. The peptide was 100% conserved in human, rat and mouse protein. The peptide was mod...

Read More

Thank you for your enquiry. We will not be able to provide the exact immunogen sequence unless I can convince the originating laboratory to release it. (They consider it proprietary information). We do know that the immunogen is from the the N-terminal...

Read More

Thank you for contacting us. The exact sequence of the immunogen is considred proprietary but there is 100% homology with all three of the species, mouse, rat and human.


I hope this information is helpful to you. Please do not hesitate to...

Read More

Thank you for contacting Abcam. The antibody epitope was form the N-terminal end, the 21 aa peptide sequence lies between amino acids********of the RICH2 protein (human).  Therefore I am unsure as to why this antibody cannot detect your C-terminal dele...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"