• Product nameAnti-CHFR antibody
    See all CHFR primary antibodies
  • Description
    Mouse monoclonal to CHFR
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: TSGAGAGRGA DPRVPPSSPA TQVCFEEPQP STSTSDLFPT ASASSTEPSP AGRERSSSC, corresponding to amino acids 136-193 of Human CHFR, with a 26kDa tag

  • Positive control
    • CHFR-transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab77225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ELISA Use at an assay dependent dilution.


  • FunctionE3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
  • Tissue specificityUbiquitous.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the CHFR family.
    Contains 1 FHA domain.
    Contains 1 PBZ-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Developmental stageWeakly expressed in G1 phase, and highly expressed during S phase.
  • DomainThe PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
    The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.
  • Post-translational
    Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Autoubiquitinated; may regulate its cellular level.
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
  • Cellular localizationNucleus > PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • Checkpoint with forkhead and ring finger domains antibody
    • Checkpoint with forkhead and RING finger domains protein antibody
    • Chfr antibody
    • CHFR_HUMAN antibody
    • E3 ubiquitin protein ligase CHFR antibody
    • E3 ubiquitin-protein ligase CHFR antibody
    • EC 6.3.2. antibody
    • FLJ10796 antibody
    • FLJ33629 antibody
    • RING finger protein 196 antibody
    • RNF 116 antibody
    • RNF 196 antibody
    • RNF116 antibody
    • RNF196 antibody
    • Ubiquitin ligase protein antibody
    see all

Anti-CHFR antibody images

  • Anti-CHFR antibody (ab77225) at 5 µg/ml + immunogen at 0.2 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CHFR antibody (ab77225) at 5 µg/ml

    Lane 1 : CHFR transfected lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-CHFR antibody (ab77225)

ab77225 has not yet been referenced specifically in any publications.

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