Overview

  • Product name
    Anti-CHFR antibody - N-terminal
    See all CHFR primary antibodies
  • Description
    Rabbit polyclonal to CHFR - N-terminal
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CHFR (N terminal). The exact sequence is proprietary.
    Database link: Q96EP1

  • Positive control
    • ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function
    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
  • Tissue specificity
    Ubiquitous.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the CHFR family.
    Contains 1 FHA domain.
    Contains 1 PBZ-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Developmental stage
    Weakly expressed in G1 phase, and highly expressed during S phase.
  • Domain
    The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
    The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.
  • Post-translational
    modifications
    Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Autoubiquitinated; may regulate its cellular level.
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
  • Cellular localization
    Nucleus > PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • Checkpoint with forkhead and ring finger domains antibody
    • Checkpoint with forkhead and RING finger domains protein antibody
    • Chfr antibody
    • CHFR_HUMAN antibody
    • E3 ubiquitin protein ligase CHFR antibody
    • E3 ubiquitin-protein ligase CHFR antibody
    • EC 6.3.2. antibody
    • FLJ10796 antibody
    • FLJ33629 antibody
    • RING finger protein 196 antibody
    • RNF 116 antibody
    • RNF 196 antibody
    • RNF116 antibody
    • RNF196 antibody
    • Ubiquitin ligase protein antibody
    see all

Images

  • 4% paraformaldehyde-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for CHFR (green) using ab227536 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

References

ab227536 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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