Overview

  • Product name
    Anti-CHI3L1 antibody [AT2C10]
    See all CHI3L1 primary antibodies
  • Description
    Mouse monoclonal [AT2C10] to CHI3L1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 22-383 of Human CHI3L1, purified from E. coli

  • Positive control
    • HepG2 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    AT2C10
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1500. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
  • Tissue specificity
    Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
  • Involvement in disease
    A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 18 family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39 kDa synovial protein antibody
    • ASRT7 antibody
    • Cartilage glycoprotein 39 antibody
    • CGP-39 antibody
    • CGP39 antibody
    • CH3L1_HUMAN antibody
    • CHI3L1 antibody
    • chitinase 3 like 1 (cartilage glycoprotein 39) antibody
    • chitinase 3 like 1 antibody
    • Chitinase 3 like protein 1 precursor antibody
    • chitinase antibody
    • Chitinase-3-like protein 1 antibody
    • Chondrocyte protein YKL40 antibody
    • GP 39 antibody
    • GP-39 antibody
    • GP39 antibody
    • HC gp39 antibody
    • HCGP 3P antibody
    • hCGP-39 antibody
    • HCgp39 antibody
    • YKL 40 antibody
    • YKL-40 antibody
    • YKL40 antibody
    • YYL 40 antibody
    see all

Images

  • IHC image of CHI3L1 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86428, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-CHI3L1 antibody [AT2C10] (ab86428) at 1/250 dilution + HepG2 cell lysates at 35 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

ab86428 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86428.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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