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Synthetic peptide within Human CHI3L2 aa 21-50 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q15782
Our Abpromise guarantee covers the use of ab171409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 44 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling CHI3L2 with ab171409 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labeling CHI3L2 with ab171409 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab171409 has not yet been referenced specifically in any publications.