Chicken Anti-Rabbit IgG H&L (TRITC) (ab6826)

Overview

  • Product nameChicken Anti-Rabbit IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Description
    Chicken polyclonal Secondary Antibody to Rabbit IgG - H&L (TRITC )
  • Target speciesRabbit
  • Tested applicationsImmunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notesTetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notessuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • References for Chicken Anti-Rabbit IgG H&L (TRITC) (ab6826)

    ab6826 has not yet been referenced specifically in any publications.

    Product Wall

    I'm sorry to hear you are experiencing problems with ab6826, the fact that a "no primary" control gave you high background does indeed point towards a non-specificity of a damaged vial. Could you please clarify what dilution of this secondary antibody ...

    Read More

    All the information avaliable for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose". Therefore this antibody will be...

    Read More

    There should be no difference between any of these secondaries; they will all work similarly well. However, if you wish to reduce background with the human cells then we suggest using secondaries that have been pre-adsorbed against human serum, thus, ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"