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Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.
|Components||24 tests||48 tests|
|100X Protease Inhibitor Cocktail||1 x 25µl||1 x 50µl|
|5X Lysis Buffer I||1 x 12ml||1 x 24ml|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Antibody Buffer||1 x 15ml||1 x 30ml|
|Anti-H3K9me2 (1 mg/mL)||1 x 5µl||1 x 8µl|
|Binding Buffer||1 x 5ml||1 x 8ml|
|ChIP Dilution Buffer||1 x 2ml||1 x 6ml|
|DNA Release Buffer||1 x 2ml||2 x 2ml|
|Elution Buffer||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Lysis Buffer II||1 x 3ml||1 x 6ml|
|Lysis Buffer III||1 x 2ml||1 x 4ml|
|Lysis Buffer IV||1 x 1.5ml||1 x 5ml|
|Normal Mouse IgG (1 mg/mL)||1 x 10µl||1 x 10µl|
|Proteinase K (10 mg/mL)||1 x 25µl||1 x 50µl|
|Reverse Buffer||1 x 2ml||2 x 2ml|
|Wash Buffer||1 x 28ml||2 x 28ml|
Our Abpromise guarantee covers the use of ab117137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|