Overview

  • Product nameAnti-Chitinase 3 like protein 3 antibody
  • Description
    Rabbit polyclonal to Chitinase 3 like protein 3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Chitinase 3 like protein 3.

  • Positive control
    • This antibody gave a positive signal in Mouse bone marrow and spleen tissue lysates. It also gave a positive signal in RAW 246.7 cells in IF/ICC.

Properties

Applications

Our Abpromise guarantee covers the use of ab93034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceChitinase-3-like protein 3 (Ym1) is a lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine; binding to oligomeric saccharides is much stronger. It also binds chitin and heparin. This protein has been implicated in inflammation and allergy.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • YM1 antibody

Anti-Chitinase 3 like protein 3 antibody images

  • ICC/IF image of ab93034 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93034 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Chitinase 3 like protein 3 antibody (ab93034) at 1 µg/ml

    Lane 1 : Mouse Bone Marrow Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 26 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-Chitinase 3 like protein 3 antibody (ab93034)

This product has been referenced in:
  • Katsuyama E  et al. Interleukin-1 receptor-associated kinase-4 (IRAK4) promotes inflammatory osteolysis by activating osteoclasts and inhibiting formation of foreign body giant cells. J Biol Chem 290:716-26 (2015). WB ; Mouse . Read more (PubMed: 25404736) »
  • Cudejko C  et al. p16INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages. Blood 118:2556-66 (2011). IHC ; Mouse . Read more (PubMed: 21636855) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer with 0.05% tween 20
Sample Mouse Tissue sections (lung)
Specification lung
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Cervelli

Verified customer

Submitted Sep 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"