Overview

  • Product nameAnti-Chk1 antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1
  • Tested applicationsSuitable for: IHC-P, ICC, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human). Epitope is in the region between 250 and 300 of Chk1 (Swiss-prot: O14757).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab17191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/500 - 1/2000.

Formaldehyde fixation with Triton X 100 permeabilization is recommended.

WB 1/10000 - 1/25000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP Use a concentration of 1 - 3 µg/ml.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Anti-Chk1 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells treated with neocarzinostatin labelling Chk1 with ab17191 at 1/1000 (1µg/ml) . A FITC-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.


  • Predicted band size : 54 kDa

    Samples: whole cell lysate (20 micrograms (mcg)) from Hela cells.

    ab17191 was used at the concentrations indicated on the image.

    Samples: whole cell lysate (20 micrograms (mcg)) from Hela cells.

    ab17191 was used at the concentrations indicated on the image.

References for Anti-Chk1 antibody (ab17191)

ab17191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"