Overview

  • Product nameAnti-Chk1 antibody
    See all Chk1 primary antibodies
  • Description
    Goat polyclonal to Chk1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic phospho peptide (Human), which represents a portion of human Chk 1 encoded within exon 8.

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Anti-Chk1 antibody images



  • Predicted band size : 54 kDa


    ab2845 at a 1:10000 dilution staining ~57 kDa Chk 1 in HeLa whole cell lysate (30µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds exposure).
  • All lanes : Anti-Chk1 antibody (ab2845) at 1/10000 dilution

    Lane 1 : Whole cell lysate of HT29 tumour cell line, untreated
    Lane 2 : Whole cell lysate of HT29 tumour cell line, treated with DMSO
    Lane 3 : Whole cell lysate of HT29 tumour cell line, treated with 50nM SN38 for 6h

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated cow anti-goat antibody at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Chk1 antibody (ab2845)

This product has been referenced in:
  • Nawrocki ST  et al. The NEDD8-activating enzyme inhibitor MLN4924 disrupts nucleotide metabolism and augments the efficacy of cytarabine. Clin Cancer Res 21:439-47 (2015). Human . Read more (PubMed: 25388161) »
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT29 tumour cell line)
Loading amount 50 µg
Specification HT29 tumour cell line
Treatment 50nM SN38 for 6h
Gel Running Conditions Reduced Denaturing (10% SDS page tris glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jun 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"