Overview

  • Product name
    Anti-Chk1 antibody [AF7H4]
    See all Chk1 primary antibodies
  • Description
    Mouse monoclonal [AF7H4] to Chk1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    His-tagged recombinant full length Human protein, purified from E.coli

  • Positive control
    • Lysates from HeLa, 293T, RD, C6 or L929 cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR36933 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab80615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP Use at an assay dependent concentration.

Use at 2μg

Target

Images

  • All lanes : Anti-Chk1 antibody [AF7H4] (ab80615) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : RD cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : L929 cell lysate


    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References

This product has been referenced in:
  • Osorio-Zambrano WF & Davey S Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling. PLoS One 10:e0144434 (2015). Read more (PubMed: 26658951) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80615.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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