Overview

  • Product nameAnti-Chk1 antibody [E250]
    See all Chk1 primary antibodies
  • Description
    Rabbit monoclonal [E250] to Chk1
  • Specificityab32531 recognises Chk1.
  • Tested applicationsSuitable for: WB, ICC, IPmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to N-terminal residues of human Chk1

  • Positive control
    • K562 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC 1/50.
IP Use at an assay dependent dilution.
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    Anti-Chk1 antibody [E250] images

    • Anti-Chk1 antibody [E250] (ab32531) at 1/1000 dilution + K562 cell lysate

      Predicted band size : 54 kDa
      Observed band size : 54 kDa

    References for Anti-Chk1 antibody [E250] (ab32531)

    This product has been referenced in:
    • Luo MH  et al. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol 81:1934-50 (2007). WB ; Human . Read more (PubMed: 17151099) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Jun 30 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton in PBS
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 09 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"