Recombinant
RabMAb

Anti-Chk1 antibody [EP691Y] (ab40866)

Overview

  • Product name
    Anti-Chk1 antibody [EP691Y]
    See all Chk1 primary antibodies
  • Description
    Rabbit monoclonal [EP691Y] to Chk1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Chk1 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa cells lysate and human breast carcinoma.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Images

  • Immunofluorescence staining of MCF7 cells with purified ab40866 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Overlay histogram showing HeLa cells stained with ab40866 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40866, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-Chk1 antibody [EP691Y] (ab40866) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunohistochemical analysis of Chk1 expression in paraffin-embedded human breast carcinoma using ab40866 at a 1:250 dilution.

References

This product has been referenced in:
  • Lunardi A  et al. Suppression of CHK1 by ETS Family Members Promotes DNA Damage Response Bypass and Tumorigenesis. Cancer Discov 5:550-63 (2015). IHC ; Human . Read more (PubMed: 25653093) »
  • Okita N  et al. CHK1 cleavage in programmed cell death is intricately regulated by both caspase and non-caspase family proteases. Biochim Biophys Acta 1830:2204-13 (2013). Read more (PubMed: 23085068) »

See all 6 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6.0
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 03 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HCT116)
Specification
HCT116
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human colon cancer cells)
Loading amount
25 µg
Specification
human colon cancer cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cell)
Loading amount
50000 cells
Specification
Human mammary cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human breast adenocarcinoma cell line (MCF7))
Loading amount
35 µg
Specification
human breast adenocarcinoma cell line (MCF7)
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cell line)
Loading amount
20 µg
Specification
Colon cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2011

Thank you for your enquiry. Unfortunately, based on the sequence alignment, this anti-chk1 will not work for s. pombe samples. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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