Anti-Chk1 (phospho S280) antibody (ab47560)

Overview

  • Product nameAnti-Chk1 (phospho S280) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S280)
  • SpecificityThis antibody detects endogenous levels of Chk1 only when phosphorylated at serine 280.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 280 (V-T-SP-G-G).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab47560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
ELISA 1/20000.

Target

Anti-Chk1 (phospho S280) antibody images

  • All lanes : Anti-Chk1 (phospho S280) antibody (ab47560) at 1/500 dilution

    Lane 1 : HT29 cells, untreated
    Lane 2 : HT29 cells treated with UV


    Predicted band size : 54 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-Chk1 (phospho S280) antibody (ab47560)

ab47560 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"