Anti-Chk1 (phospho S280) antibody (ab59988)

Overview

  • Product nameAnti-Chk1 (phospho S280) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S280)
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acid residues surrounding S280 region of human Chk1, conjugated to KLH

  • Positive control
    • A2058, Ramos, Jurkat, HL60, Hela cells or mouse brain lysates. Human hepatocarcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesAfter protein G affinity chromatography, the antibody fraction was peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Anti-Chk1 (phospho S280) antibody images

  • All lanes : Anti-Chk1 (phospho S280) antibody (ab59988) at 1/100 dilution

    Lane 1 : A2058 cell lysate
    Lane 2 : mouse brain lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HL60 cell lysate
    Lane 6 : Hela cell lysate


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab59988 at 1/50-1/100 dilution staining Chk1 by Immunohistochemistry on Formalin-fixed, Paraffin-embedded Human hepatocarcinoma tissue.
  • ab59988 staining phosphoChk1 (S280) in human colon adenocarcinoma cells (LoVo) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% and blocked with 1% BSA for 2 hours at 20°C. Samples were incubated with primary antibody (1/250 in PBS + 0.1% Triton) for 3 hours at 20°C. A 1.4 nm Gold (ultra-small) FITC-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

References for Anti-Chk1 (phospho S280) antibody (ab59988)

ab59988 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HCT116)
Specification HCT116
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

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Submitted Dec 17 2013

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Application Western blot
Sample Human Tissue lysate - whole (human colon cancer cells)
Loading amount 25 µg
Specification human colon cancer cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Apr 22 2013

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Application Western blot
Sample Human Cell lysate - whole cell (Human mammary cells)
Loading amount 50000 cells
Specification Human mammary cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Ms. pierre laetitia

Verified customer

Submitted Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cancer mammary cell)
Specification cancer mammary cell
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0,5%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human colon adenocarcinoma cells (LoVo))
Specification Human colon adenocarcinoma cells (LoVo)
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.1%
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Sep 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human colon adenocarcinoma cells (LoVo))
Loading amount 20 µg
Specification Human colon adenocarcinoma cells (LoVo)
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 19 2011

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Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 35 µg
Specification MCF7
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Feb 14 2011

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Application Western blot
Sample Human Cell lysate - whole cell (Colon cell line)
Loading amount 20 µg
Specification Colon cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

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Submitted Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"