Anti-Chk1 (phospho S286) antibody (ab193234)

Overview

  • Product name
    Anti-Chk1 (phospho S286) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S286)
  • Host species
    Rabbit
  • Specificity
    ab193234 detects endogenous level of Chk1 only when phosphorylated at serine 286.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Chk1 (phospho S286) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of serine 286 (S-E-S(p)-P-S) .
    Database link: O14757

  • Positive control
    • K562 cell lysate, treated with Na3VO4 (Sodium orthovanadate).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab193234 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Images

  • All lanes : Anti-Chk1 (phospho S286) antibody (ab193234) at 1/500 dilution

    Lane 1 : K562 cell lysate, treated with Na3VO4
    Lane 2 : K562 cell lysate, treated with Na3VO4 with antigen-specific peptide

    Predicted band size: 54 kDa

References

ab193234 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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