Anti-Chk1 (phospho S286) antibody (ab62243)

Overview

  • Product name
    Anti-Chk1 (phospho S286) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S286)
  • Specificity
    ab62243 detects endogenous levels of Chk1 only when phosphorylated at serine 286.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 286 (S-E-SP-P-S).

  • Positive control
    • HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab62243 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Images

  • ab62243 at 1/500 - 1/1000 dilution staining Chk1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

ab62243 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cell)
Loading amount
50000 cells
Specification
Human mammary cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (colon cell line)
Loading amount
25 µg
Specification
colon cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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