Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)


  • Product nameAnti-Chk1 (phospho S296) antibody [EPR915]
    See all Chk1 primary antibodies
  • Description
    Rabbit monoclonal [EPR915] to Chk1 (phospho S296)
  • SpecificityThe antibody only detects Chk1 phosphorylated at Serine 296.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 296 of human Chk1

  • Positive control
    • 283T cell lysate treated with UV and AP; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab79758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

For unpurified use at 1/1000 - 1/2000

  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • C85740 antibody
      • Cell cycle checkpoint kinase antibody
      • Checkpoint , S. pombe, homolog of, 1 antibody
      • Checkpoint kinase 1 antibody
      • Checkpoint kinase 1 homolog (S. pombe) antibody
      • CHEK 1 antibody
      • Chek1 antibody
      • Chk 1 antibody
      • Chk1 antibody
      • CHK1 checkpoint homolog (S. pombe) antibody
      • CHK1_HUMAN antibody
      • EC antibody
      • rad27 antibody
      • Serine/threonine protein kinase Chk1 antibody
      • Serine/threonine-protein kinase CHK1 antibody
      • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
      see all

    Anti-Chk1 (phospho S296) antibody [EPR915] images

    • All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution

      Lane 1 : Untreated 293 cell lysate
      Lane 2 : Treated with Calyculin A 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 54 kDa
    • All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)

      Lane 1 : Untreated 293 cell Lysate
      Lane 2 : Treated with Calyculin 293 cell Lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 54 kDa

    References for Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)

    This product has been referenced in:
    • Bryant C  et al. Inhibition of the checkpoint kinase Chk1 induces DNA damage and cell death in human Leukemia and Lymphoma cells. Mol Cancer 13:147 (2014). WB . Read more (PubMed: 24913641) »
    • Rawlinson R & Massey AJ ?H2AX and Chk1 phosphorylation as predictive pharmacodynamic biomarkers of Chk1 inhibitor-chemotherapy combination treatments. BMC Cancer 14:483 (2014). WB ; Human . Read more (PubMed: 24996846) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (colon cell line)
    Loading amount 20 µg
    Specification colon cell line
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted Dec 29 2010