Anti-Chk1 (phospho S301) antibody (ab61023)

Overview

  • Product name
    Anti-Chk1 (phospho S301) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S301)
  • Specificity
    ab61023 detects endogenous levels of Chk1 only when phosphorylated at serine 301.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).

  • Positive control
    • Extracts from 293 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

Target

Anti-Chk1 (phospho S301) antibody images

  • All lanes : Anti-Chk1 (phospho S301) antibody (ab61023) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with Immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 50,~100 kDa (why is the actual band size different from the predicted?)

References for Anti-Chk1 (phospho S301) antibody (ab61023)

ab61023 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cell)
Loading amount
50000 cells
Specification
Human mammary cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cell line)
Loading amount
20 µg
Specification
Colon cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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