Anti-Chk1 (phospho S317) antibody (ab38518)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S317) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Chk1 aa 250-350 (phospho S317).
Database link: O14757 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
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Alternative names
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
Images
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All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution
Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
Lane 2 : Extracts from COLO cells (5-30ug of total protein)
Secondary
All lanes : Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)
Predicted band size: 54 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab38518 has been referenced in 2 publications.
- Jiang D et al. TET3-mediated DNA oxidation promotes ATR-dependent DNA damage response. EMBO Rep 18:781-796 (2017). PubMed: 28325772
- Kadaja M et al. Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses. PLoS Pathog 5:e1000397 (2009). ICC/IF ; Human . PubMed: 19390600