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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 150% Sodium chloride, PBS (without Mg2+ and Ca2+), pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: 1/4000.
ICC/IF: Use at an assay dependent dilution (PMID 19390600).
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Information by UniProt
Cell cycle checkpoint kinase antibody
Checkpoint , S. pombe, homolog of, 1 antibody
Anti-Chk1 (phospho S317) antibody images
Western blot - Chk1 (phospho S317) antibody (ab38518)
All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution Lane 1 : Extracts from MCF7 cells (5-30ug of total protein) Lane 2 : Extracts from COLO cells (5-30ug of total protein) Secondary Alkaline Phosphatase Goat Anti Rabbit IgG (H+L) Predicted band size : 54 kDa Observed band size : 63 kDa ( why is the actual band size different from the predicted?)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"