Overview

  • Product nameAnti-Chk1 (phospho S317) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S317)
  • SpecificityChk1(Phospho-Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 150% Sodium chloride, PBS (without Mg2+ and Ca2+), pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
  • Application notesELISA: 1/4000.
    ICC/IF: Use at an assay dependent dilution (PMID 19390600).
    WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • C85740 antibody
      • Cell cycle checkpoint kinase antibody
      • Checkpoint , S. pombe, homolog of, 1 antibody
      • Checkpoint kinase 1 antibody
      • Checkpoint kinase 1 homolog (S. pombe) antibody
      • CHEK 1 antibody
      • Chek1 antibody
      • Chk 1 antibody
      • Chk1 antibody
      • CHK1 checkpoint homolog (S. pombe) antibody
      • CHK1_HUMAN antibody
      • EC 2.7.11.1 antibody
      • rad27 antibody
      • Serine/threonine protein kinase Chk1 antibody
      • Serine/threonine-protein kinase CHK1 antibody
      • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
      see all

    Anti-Chk1 (phospho S317) antibody images

    • All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution

      Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
      Lane 2 : Extracts from COLO cells (5-30ug of total protein)

      Secondary
      Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)

      Predicted band size : 54 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)

    References for Anti-Chk1 (phospho S317) antibody (ab38518)

    This product has been referenced in:
    • Kadaja M  et al. Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses. PLoS Pathog 5:e1000397 (2009). ICC/IF ; Human . Read more (PubMed: 19390600) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human mammary cells)
    Loading amount 50000 cells
    Specification human mammary cells
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Ms. pierre laetitia

    Verified customer

    Submitted Feb 23 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hela cells)
    Loading amount 10 µg
    Specification Hela cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted May 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"