Anti-Chk1 (phospho S317) antibody (ab59239)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S317) -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; Ramos. IHC-P: Human testis FFPE tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
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Alternative names
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
Images
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All lanes : Anti-Chk1 (phospho S317) antibody (ab59239) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Chk1 (phospho S317) peptide (ab94392) at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Chk1 (phospho S317) peptide (ab94392) at 1 µg/ml
Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate with Chk1 (phospho S317) peptide (ab94392) at 1 µg/ml
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Chk1 peptide (ab116584) at 1 µg/ml
Lane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Chk1 peptide (ab116584) at 1 µg/ml
Lane 9 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate with Chk1 peptide (ab116584) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa (possible post-translational modification)
Exposure time: 20 minutes -
IHC image of Chk1 (phospho S317) staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59239, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ICC/IF image of ab59239 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59239, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, MCF-7 cells at 5µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab59239 has been referenced in 2 publications.
- Shi J et al. MTA2 sensitizes gastric cancer cells to PARP inhibition by induction of DNA replication stress. Transl Oncol 14:101167 (2021). PubMed: 34280886
- Nishimoto K et al. HDAC3 Is Required for XPC Recruitment and Nucleotide Excision Repair of DNA Damage Induced by UV Irradiation. Mol Cancer Res 18:1367-1378 (2020). PubMed: 32527949