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The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).
Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.
Our Abpromise guarantee covers the use of ab47318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200. See Abreview.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20978077Fixed in -20°C methanol.|
|IHC-P||1/50 - 1/100.|