Anti-Chk1 (phospho S345) antibody (ab47318)


  • Product nameAnti-Chk1 (phospho S345) antibody
    See all Chk1 primary antibodies
  • Description
    Rabbit polyclonal to Chk1 (phospho S345)
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).

  • Positive control
    • Human breast carcinoma tissue
  • General notes

    Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Primary antibody notesChk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab47318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. See Abreview.
IHC-Fr Use at an assay dependent concentration. PubMed: 20978077Fixed in -20°C methanol.
IHC-P 1/50 - 1/100.
ELISA 1/10000.


Anti-Chk1 (phospho S345) antibody images

  • ab47318 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • ab47318 (1/200) detecting Chk1 in HeLa cells in conjunction with a goat anti-rabbit secondary antibody conjugated to Cy3® (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue), treated with Bleomycin and incubated with an antibody against Histone H2AX in order to create and expose DNA double strand breaks (green). Please refer to abreviews for further details.

    See Abreview

References for Anti-Chk1 (phospho S345) antibody (ab47318)

This product has been referenced in:
  • Wang CY  et al. DNA-PK/Chk2 induces centrosome amplification during prolonged replication stress. Oncogene N/A:N/A (2014). Read more (PubMed: 24662822) »
  • Zhou J  et al. A kinome screen identifies checkpoint kinase 1 (CHK1) as a sensitizer for RRM1-dependent gemcitabine efficacy. PLoS One 8:e58091 (2013). Human . Read more (PubMed: 23483975) »

See all 8 Publications for this product

Product Wall

Thanks for your email.
Please feel free to call me between hours of 8:30am and 5pm EST. Our Scientific Support is available from 8am - 8pm EST and you can feel free to speak to any of my colleagues about these issues as well.
I look forward t...

Read More

Thank you for completing our recent survey. As you have noted technical difficulties with two antibodies from Abcam, I just wanted to follow up to see how I might help to rectify any issues.
To help me assist you most efficiently, it would be help...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount 15 µg
Specification MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Darren Arbon

Verified customer

Submitted Apr 14 2011

Thank you for your enquiry. All the information that we have about the protocol used by K. McManus is already stated on the review. At Abcam, we always give our customers all the information that we have at hand and I am afraid that there is no othe...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde

Dr. Kirk McManus

Verified customer

Submitted Apr 19 2007