Overview

  • Product nameAnti-Chk2 antibody [DCS-270]
    See all Chk2 primary antibodies
  • Description
    Mouse monoclonal [DCS-270] to Chk2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant Human Chk2.

  • Positive control
    • HeLa cell lysates; Human intestine tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 µg/ml. Predicted molecular weight: 61 kDa.
IP Use a concentration of 6.6 - 10 µg/ml.
IHC-P Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • Tissue specificityHigh expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • Involvement in diseaseDefects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by PLK4.
  • Cellular localizationNucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

Anti-Chk2 antibody [DCS-270] images

  • All lanes : Anti-Chk2 antibody [DCS-270] (ab115149) at 0.2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : NIH3T3 cell lysate
    Lane 3 : Rat1 cell lysate

    Developed using the ECL technique

    Predicted band size : 61 kDa
  • Detection of Chk2 by Western Blot of Immunprecipitate.
    ab115149 at 0.2µg/ml staining Chk2 in HeLa cell lysate immunoprecipitated using anti-Chk2 at 10ug/ml (lane 2). Lane 1: IgG control.
  • ab115149 at 5ug/ml staining Chk2 in Paraffin-embedded Human intestine tissue by Immunohistochemistry.

References for Anti-Chk2 antibody [DCS-270] (ab115149)

ab115149 has not yet been referenced specifically in any publications.

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