Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] (ab68267)

Overview

  • Product nameAnti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y]
    See all Chk2 primary antibodies
  • Description
    Rabbit monoclonal [EPR916(2)Y] to Chk2 (phospho S33 + S35)
  • SpecificityThis antibody detects Chk2 phosphorylated on Serine 33/35.
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Serine 33/35 of human Chk2.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notesICC: 1/100 - 1/250.
    WB: 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    Is unsuitable for Flow Cyt, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
    • Tissue specificityHigh expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
    • Involvement in diseaseDefects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated by PLK4.
    • Cellular localizationNucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDS 1 antibody
      • Cds1 antibody
      • Cds1 homolog antibody
      • Checkpoint kinase 2 antibody
      • Checkpoint like protein CHK2 antibody
      • CHEK 2 antibody
      • Chek2 antibody
      • Chk 2 antibody
      • CHK2 checkpoint homolog (S. pombe) antibody
      • CHK2 checkpoint homolog antibody
      • CHK2_HUMAN antibody
      • hCds1 antibody
      • HuCds 1 antibody
      • LFS 2 antibody
      • LFS2 antibody
      • PP1425 antibody
      • RAD 53 antibody
      • RAD53 antibody
      • Rad53 homolog antibody
      • Serine/threonine protein kinase Chk2 antibody
      • Serine/threonine-protein kinase Chk2 antibody
      see all

    Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] images

    • All lanes : Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] (ab68267) at 1/500 dilution

      Lane 1 : HeLa cell lysate, untreated.
      Lane 2 : HeLa cell lysate, treated with irradiation.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 61 kDa
      Observed band size : 61 kDa

    References for Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] (ab68267)

    This product has been referenced in:
    • Guo X  et al. Interdependent phosphorylation within the kinase domain T-loop Regulates CHK2 activity. J Biol Chem 285:33348-57 (2010). WB . Read more (PubMed: 20713355) »

    See 1 Publication for this product

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