Anti-Chk2 (phospho T383) antibody (ab59408)

Overview

  • Product name
    Anti-Chk2 (phospho T383) antibody
    See all Chk2 primary antibodies
  • Description
    Rabbit polyclonal to Chk2 (phospho T383)
  • Specificity
    Detects Human Chk2 only when phosphorylated at threonine 383 (Mouse: Thr387; Rat: Thr386)
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 383 (M-R-TP-L-C).

  • Positive control
    • COS7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab59408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ELISA 1/20000.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • Tissue specificity
    High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • Involvement in disease
    Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by PLK4.
  • Cellular localization
    Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

Anti-Chk2 (phospho T383) antibody images

  • All lanes : Anti-Chk2 (phospho T383) antibody (ab59408) at 1/500 dilution

    Lane 1 : COS7 cell extracts, UV treated (30 mins)
    Lane 2 : COS7 cell extracts, UV treated (30 mins) with immunising phosphopeptide


    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk2 (phospho T383) (green) using ab59408 at 1/100 dilution in ICC/IF, in the presence (right panel) or absence (left panel) of immunizing phosphopetide.

  • ab59408 staining Chk2 (phospho T383) in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 37°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References for Anti-Chk2 (phospho T383) antibody (ab59408)

This product has been referenced in:
  • Petsalaki E & Zachos G Chk2 prevents mitotic exit when the majority of kinetochores are unattached. J Cell Biol 205:339-56 (2014). Read more (PubMed: 24798733) »
  • Liu C  et al. Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adult rats through estrogen-like activity. Cell Death Dis 4:e676 (2013). WB . Read more (PubMed: 23788033) »

See all 4 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Human Cell (Colon carcinoma cells)
Specification
Colon carcinoma cells
Permeabilization
Yes - 0.5% Triton
Fixative
Paraformaldehyde
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Mar 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (Human Colon carcinoma BE cells)
Specification
Human Colon carcinoma BE cells
Treatment
Etoposide 10 μM for 8 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Jan 28 2013

Vielen Dank für das Senden des ausgefüllten Fragebogens.

Ich habe mir das Protokoll auch mit dem Labor angeschaut.

Das Labor hat keineBanden bei100 oder 150 kD erhalten. Es könnte jedoch sein, dass je nach Probenzuber...

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Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habeden besprochnenFragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des ...

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Application
Western blot
Sample
Xenopus laevis Cell lysate - other (One egg or embryo equivalent loaded per lane)
Loading amount
1 cells
Specification
One egg or embryo equivalent loaded per lane
Treatment
100 µM Bleomycin for 18 hours
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Thomas Mcgarry

Verified customer

Submitted Jan 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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