Human Predicted to work with:
Mouse, Rat, Cow, Pig
Synthetic peptide within Human Chk2 (phospho T68). The exact sequence is proprietary. The synthetic peptide corresponding to amino acids residues surrounding the phospho-threonine 68 of Human Chk2. Database link: O96017
U2OS whole cell lysate/extract UVC-treated at 50 mJ and recovered for 2 or 6 hours
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
Involvement in disease
Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. Contains 1 FHA domain. Contains 1 protein kinase domain.
Phosphorylated by PLK4.
Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
Western blot - Anti-Chk2 (phospho T68) antibody (ab183895)
All lanes : Anti-Chk2 (phospho T68) antibody (ab183895) at 1/250 dilution
Lane 1 : Untreated U2OS whole cell lysate/extract Lane 2 : U2OS whole cell lysate/extract UVC-treated at 50 mJ and recovered for 2 hours Lane 3 : U2OS whole cell lysate/extract UVC-treated at 50 mJ and recovered for 6 hours