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Our Abpromise guarantee covers the use of ab3501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).|
The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.
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