Overview

  • Product nameAnti-Chk2 (phospho T68) antibody [Y171]
    See all Chk2 primary antibodies
  • Description
    Rabbit monoclonal [Y171] to Chk2 (phospho T68)
  • SpecificityThe antibody will detect Chk2 phosphorylation on Threonine 68. This antibody may also detect all splice isoforms (2-12) based on sequence homology.
  • Tested applicationsSuitable for: IP, WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Threonine 68 of human Chk2

  • Positive control
    • Untreated HEK-293 cell lysates, cell lysates from HEK-293 cells treated with UV and/or phosphatase.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
WB 1/1000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 61 kDa).
Dot blot 1/500.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • FunctionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
    • Tissue specificityHigh expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
    • Involvement in diseaseDefects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated by PLK4.
    • Cellular localizationNucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDS 1 antibody
      • Cds1 antibody
      • Cds1 homolog antibody
      • Checkpoint kinase 2 antibody
      • Checkpoint like protein CHK2 antibody
      • CHEK 2 antibody
      • Chek2 antibody
      • Chk 2 antibody
      • CHK2 checkpoint homolog (S. pombe) antibody
      • CHK2 checkpoint homolog antibody
      • CHK2_HUMAN antibody
      • hCds1 antibody
      • HuCds 1 antibody
      • LFS 2 antibody
      • LFS2 antibody
      • PP1425 antibody
      • RAD 53 antibody
      • RAD53 antibody
      • Rad53 homolog antibody
      • Serine/threonine protein kinase Chk2 antibody
      • Serine/threonine-protein kinase Chk2 antibody
      see all

    Anti-Chk2 (phospho T68) antibody [Y171] images

    • All lanes : Anti-Chk2 (phospho T68) antibody [Y171] (ab32148) at 1/5000 dilution

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours.
      Lane 3 : Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours. Membrane incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 61 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Blocking/dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Chk2 (phospho T68) antibody [Y171] (ab32148) at 1/1000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : 293 cell lysate treated with 2uM Doxorubicin
      Lane 3 : 293 cell lysate treated with 10uM Doxorubicin


      Predicted band size : 61 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    • Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-Chkr2 (pT68) RabMAb (ab32148) dilution 1:500
    • All lanes : Anti-Chk2 (phospho T68) antibody [Y171] (ab32148) at 1/1000 dilution

      Lane 1 : HCT116 treated with DMSO (control) whole cell lysate
      Lane 2 : HCT116 treated with 10 µM etoposide for 24 hours whole cell lysate

      Lysates/proteins at 60 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 61 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 seconds

      Non-specific band : 35, 30 kDa

      Blocking/Diluting buffer : 5% Milk

       

      See Abreview

    References for Anti-Chk2 (phospho T68) antibody [Y171] (ab32148)

    This product has been referenced in:
    • Li WF  et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). Human . Read more (PubMed: 24728101) »
    • Zhang D  et al. Sterigmatocystin-induced DNA damage triggers G2 arrest via an ATM/p53-related pathway in human gastric epithelium GES-1 cells in vitro. PLoS One 8:e65044 (2013). WB ; Human . Read more (PubMed: 23705030) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (lymphocytes)
    Specification lymphocytes
    Treatment UV treated
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 07 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116 colon cancer cell line)
    Loading amount 60 µg
    Specification HCT116 colon cancer cell line
    Treatment DMSO control and 10 µM etoposide for 24 hrs
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    Submitted Oct 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"