Overview

  • Product name
  • Description
    Rabbit polyclonal to CHL1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 788-1025 of Human CHL1, accession number NM_006614.

  • Positive control
    • HL60 and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab106269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 135 kDa (predicted molecular weight: 137 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    CHL1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. CHL1 plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. CHL1 may play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins and recruits ANK3 to the plasma membrane.
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
  • Alternative names
    • CALL antibody
    • Cell adhesion molecule with homology to L1CAM (close homolog of L1) antibody
    • Cell adhesion molecule with homology to L1CAM (close homologue of L1) antibody
    • Cell adhesion molecule with homology to L1CAM antibody
    • Close homolog of L1 antibody
    • FLJ44930 antibody
    • L1 cell adhesion molecule 2 antibody
    • L1CAM2 antibody
    • MGC132578 antibody
    • Neural cell adhesion molecule antibody
    see all

Anti-CHL1 antibody images

  • All lanes : Anti-CHL1 antibody (ab106269) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 137 kDa

References for Anti-CHL1 antibody (ab106269)

This product has been referenced in:
  • Martín-Sánchez E  et al. CHL1 hypermethylation as a potential biomarker of poor prognosis in breast cancer. Oncotarget N/A:N/A (2017). Read more (PubMed: 28178655) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human immortalised but non-neoplastic mammary HBL-)
Permeabilization
No
Specification
Human immortalised but non-neoplastic mammary HBL-
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Esperanza Martin-Sanchez

Verified customer

Submitted Feb 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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