Human Predicted to work with:
Recombinant fragment, corresponding to amino acids 788-1025 of Human CHL1, accession number NM_006614.
HL60 and Jurkat cell lysates.
FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 135 kDa (predicted molecular weight: 137 kDa).
Use at an assay dependent concentration.
RelevanceCHL1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. CHL1 plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. CHL1 may play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins and recruits ANK3 to the plasma membrane.