Overview

  • Product name
  • Description
    Rabbit polyclonal to CHL1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse CHL1.

  • Positive control
    • This antibody gave a positive signal in the following mouse tissue lysates: Cerebellum; Spinal Cord; Retina; Optic Nerve.

Properties

Applications

Our Abpromise guarantee covers the use of ab93197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 135 kDa).

Target

  • Relevance
    CHL1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. CHL1 plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. CHL1 may play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins and recruits ANK3 to the plasma membrane.
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
  • Alternative names
    • CALL antibody
    • Cell adhesion molecule with homology to L1CAM (close homolog of L1) antibody
    • Cell adhesion molecule with homology to L1CAM (close homologue of L1) antibody
    • Cell adhesion molecule with homology to L1CAM antibody
    • Close homolog of L1 antibody
    • FLJ44930 antibody
    • L1 cell adhesion molecule 2 antibody
    • L1CAM2 antibody
    • MGC132578 antibody
    • Neural cell adhesion molecule antibody
    see all

Images

  • All lanes : Anti-CHL1 antibody (ab93197) at 1 µg/ml

    Lane 1 : Cerebellum (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Retina (Mouse) Tissue Lysate
    Lane 4 : Optic Nerve (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 135 kDa
    Observed band size : 220 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,140 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab93197 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93197.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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