Anti-Chlamydia trachomatis MOMP antibody [BIOD166] (ab20881)

Submit an Abreview Q&A (1)Specific References (2)
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Overview

  • Product name
    Anti-Chlamydia trachomatis MOMP antibody [BIOD166]
    See all Chlamydia trachomatis MOMP primary antibodies
  • Description
    Mouse monoclonal [BIOD166] to Chlamydia trachomatis MOMP
  • Specificity
    Ab20881 is specific for major outer membrane protein (MOMP) of Chlamydia trachomatis. No reactivity with C. pneumoniae.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Chlamydia trachomatis
    Does not react with: Chlamydia pneumoniae
  • Immunogen

    Chlamydia trachomatis elementary bodies.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    BIOD166
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21628561
WB Use at an assay dependent concentration. PubMed: 21628561
ELISA 1/20 - 1/200.

Target

  • Relevance
    Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell's nucleus. Chlamydia trachomatis is an intracellular organism that has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T cells, and B cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be Gram negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross linked with disulfide bonds. It also contains cysteine rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996). Chlamydia usually infects the cervix and fallopian tubes of women and the urethra of men. Chlamydial infections are believed to be one of the most common of all STDs. It is generally thought that in a population of 15 million, there are up to 300,000 cases of chlamydia each year. Thus, there are many undiagnosed cases of chlamydia in the community. It has been estimated that the true prevalence of chlamydia in the sexually active population may be in the order of 5% to 10%. Chlamydia is one of the leading causes of blindness in underdeveloped countries.
  • Cellular localization
    Outer membrane; multi pass membrane protein.
  • Database links
    see all
  • Alternative names
    • Major Outer Membrane Protein antibody
    • MOMP antibody
    • omp1 antibody
    • omp1F antibody
    • Omp1L1 antibody
    • ompA antibody
    • ompIL2 antibody
    see all

References for Anti-Chlamydia trachomatis MOMP antibody [BIOD166] (ab20881)

This product has been referenced in:
  • González E  et al. Chlamydia infection depends on a functional MDM2-p53 axis. Nat Commun 5:5201 (2014). Read more (PubMed: 25392082) »
  • Nguyen BD  et al. Lipooligosaccharide is required for the generation of infectious elementary bodies in Chlamydia trachomatis. Proc Natl Acad Sci U S A 108:10284-9 (2011). ICC/IF, WB . Read more (PubMed: 21628561) »

See all 2 Publications for this product

Publishing research using ab20881? Please let us know so that we can cite the reference in this datasheet.

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1 Abreview or Q&A

10
Votes: Highest First

using ab20881 in ELISA to detect whole cells; it is working but affinity is much less than other antibodies to Chlamydia; for example we get OD 0.5 when other antibodies produce OD 3.0; Used 3 concentrations of antibody at 5ug/ml, 1ug/ml and 0.2ug/ml a...

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I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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