Anti-Chloramphenicol Acetyltransferase antibody (ab50151)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 26 kDa.
ICC/IF Use a concentration of 10 µg/ml. 10 µg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene.

Target

  • RelevanceCAT is used as a tag in many systems and is used in many transcription assays. The enzyme is an effector of chloramphenicol resistance in bacteria.
  • Database links
    • Alternative names
      • Cat antibody

    References for Anti-Chloramphenicol Acetyltransferase antibody (ab50151)

    This product has been referenced in:
    • Gould PS  et al. Cellular mRNAs access second ORFs using a novel amino acid sequence-dependent coupled translation termination-reinitiation mechanism. RNA 20:373-81 (2014). WB . Read more (PubMed: 24412912) »
    • Stoll SJ  et al. The Transcription Factor HOXC9 Regulates Endothelial Cell Quiescence and Vascular Morphogenesis in Zebrafish via Inhibition of Interleukin 8. Circ Res 108:1367-77 (2011). WB . Read more (PubMed: 21493894) »

    See all 3 Publications for this product

    Product Wall

    Thank you for submitting an Abreview of ab50151. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Escherichia coli Cell (E.coli protein CAT expressed in HeLa cells)
    Specification E.coli protein CAT expressed in HeLa cells
    Fixative Formaldehyde
    Permeabilization Yes - Triton X100
    Username

    Abcam user community

    Verified customer

    Submitted Jun 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"