Overview

  • Product name
    Anti-Chloramphenicol antibody
    See all Chloramphenicol primary antibodies
  • Description
    Sheep polyclonal to Chloramphenicol
  • Specificity
    ab35658 is specific to Chloramphenicol (CAP, Cm).
  • Tested applications
    Suitable for: ELISA, FPIAmore details
  • Immunogen

    Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) conjugated to KLH using the diazotisation procedure.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Prepared by Ammonium Sulphate fractionation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab35658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
FPIA
  • Application notes
    ELISA: 1/4000 - 1/500000.
    ab35658 was used in a simple indirect ELISA with 400 ng of Chloramphenicol-BSA per well. The titres detailed above were elucidated using an HRP labelled secondary antibody/TMB detection system.

    FPIA: 1/1500.
    When tested by polarisation fluoroimmunoassay, the final titre dilution was found to be 0.5 ml tracer + 0.5 ml diluted antibody. Tracer is aromatic amino derivative of chloramphenicol labelled by reaction with fluorescein isothiocyanate (10 nmol/L).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Chloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment to treat bacterial conjunctivitis.

    References

    ab35658 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab35658.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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