Anti-Chloride Channel 5 antibody (ab97482)

Overview

  • Product nameAnti-Chloride Channel 5 antibody
    See all Chloride Channel 5 primary antibodies
  • Description
    Rabbit polyclonal to Chloride Channel 5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Pig
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 62-363 of Human chloride channel 5 (NP_000075)

  • Positive control
    • A431 whole cell lysate, HepG2 and Raji lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

Target

  • FunctionProton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
  • Tissue specificityKidney. Moderately expressed in aortic vascular smooth muscle and endothelial cells, and at a slightly higher level in the coronary vascular smooth muscle.
  • Involvement in diseaseHypophosphatemic rickets, X-linked recessive
    Nephrolithiasis 2
    Nephrolithiasis 1
    Low molecular weight proteinuria with hypercalciuria and nephrocalcinosis
  • Sequence similaritiesBelongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.
    Contains 2 CBS domains.
  • Post-translational
    modifications
    Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.
  • Cellular localizationGolgi apparatus membrane. Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel protein 5 antibody
    • Chloride channel voltage sensitive 5 antibody
    • Chloride transporter ClC-5 antibody
    • ClC-5 antibody
    • CLC5 antibody
    • CLCK2 antibody
    • CLCN5 antibody
    • CLCN5_HUMAN antibody
    • DENTS antibody
    • H(+)/Cl(-) exchange transporter 5 antibody
    • hCIC-K2 antibody
    • NPHL1 antibody
    • NPHL2 antibody
    • Voltage gated chloride ion channel CLCN5 antibody
    • XLRH antibody
    • XRN antibody
    see all

Anti-Chloride Channel 5 antibody images

  • Anti-Chloride Channel 5 antibody (ab97482) at 1/500 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 83 kDa

References for Anti-Chloride Channel 5 antibody (ab97482)

ab97482 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (LLCPK1)
Permeabilization Yes - 0.3% Triton in PBS
Specification LLCPK1
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Sufyan Ali Sayyed

Verified customer

Submitted Oct 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (LLCPK1)
Gel Running Conditions Reduced Denaturing (7.5)
Loading amount 50 µg
Specification LLCPK1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Sufyan Ali Sayyed

Verified customer

Submitted Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"