Anti-CHM antibody (ab10085)


  • Product name
  • Description
    Rabbit polyclonal to CHM
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 61-75 of Human CHM.

  • Positive control
    • Mouse muscle lysate.
  • General notes

    CHM binds unprenylated RAB proteins, presents it to the catalytic component B, and remains bound to it after the geranylgeranyl transfer reaction. The component a may be regenerated by transferring its prenylated RAB to a protein acceptor.



Our Abpromise guarantee covers the use of ab10085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 78.4 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.
  • Involvement in disease
    Defects in CHM are the cause of choroideremia (CHM) [MIM:303100]. An X-linked recessive disease characterized by a slowly progressive degeneration of the choroid, photoreceptors, and retinal pigment epithelium. Affected males develop night blindness in their teenage years followed by loss of peripheral vision and complete blindness at middle age. Carrier females are generally asymptomatic but funduscopic examination often shows patchy areas of chorioretinal atrophy.
  • Sequence similarities
    Belongs to the Rab GDI family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHM antibody
    • Chm antibody
    • Choroideraemia protein antibody
    • Choroideremia antibody
    • DXS540 antibody
    • FLJ38564 antibody
    • GGTA antibody
    • HSD 32 antibody
    • MGC102710 antibody
    • Rab escort protein 1 antibody
    • Rab geranylgeranyltransferase component A antibody
    • Rab proteins geranylgeranyltransferase component A 1 antibody
    • RAE1_HUMAN antibody
    • REP 1 antibody
    • REP-1 antibody
    • REP1 antibody
    • TCD antibody
    • TCD protein antibody
    see all

Anti-CHM antibody images

  • Predicted band size : 78.4 kDa

    ab10085 at 1/1000 dilution staining approximately 73kDa band of mouse CHM in mouse muscle lysate by Western blot (ECL).

References for Anti-CHM antibody (ab10085)

ab10085 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (fibroblast)
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 23 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry. This antibody has not been tested for cross reactivity with REP2. Typically, we use 85% homology as the benchmark for whether or not something will cross react. While it is possible that it may slightly cross react with REP2...

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Thank you for your phone call and I'm sorry to hear that this antibody did not work out for you. I have instructed our accounting department to issue you a refund for your order for ab10085. If you have any questions regarding the refund, please contac...

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