Overview

  • Product name
    Anti-CHM antibody - N-terminal
    See all CHM primary antibodies
  • Description
    Goat polyclonal to CHM - N-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant fragment within Rat CHM aa 1-80 (N terminal). The exact sequence is proprietary.
    Sequence:

    MADNLPSDFD VIVIGTGLPE SIIAAACSRS GQRVLHVDSR SYYGGNWASF SFSGLLSWLK EYQENNDVVT ENSMWQEQIL


    Database link: P37727

  • Positive control
    • HEK293 cell lysates transfected with VS-REP1 or AtT20

Properties

Applications

Our Abpromise guarantee covers the use of ab192454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 72 kDa.

Target

  • Function
    Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.
  • Involvement in disease
    Defects in CHM are the cause of choroideremia (CHM) [MIM:303100]. An X-linked recessive disease characterized by a slowly progressive degeneration of the choroid, photoreceptors, and retinal pigment epithelium. Affected males develop night blindness in their teenage years followed by loss of peripheral vision and complete blindness at middle age. Carrier females are generally asymptomatic but funduscopic examination often shows patchy areas of chorioretinal atrophy.
  • Sequence similarities
    Belongs to the Rab GDI family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHM antibody
    • Chm antibody
    • Choroideraemia protein antibody
    • Choroideremia antibody
    • DXS540 antibody
    • FLJ38564 antibody
    • GGTA antibody
    • HSD 32 antibody
    • MGC102710 antibody
    • Rab escort protein 1 antibody
    • Rab geranylgeranyltransferase component A antibody
    • Rab proteins geranylgeranyltransferase component A 1 antibody
    • RAE1_HUMAN antibody
    • REP 1 antibody
    • REP-1 antibody
    • REP1 antibody
    • TCD antibody
    • TCD protein antibody
    see all

Images

  • All lanes : Anti-CHM antibody - N-terminal (ab192454) at 1/1000 dilution

    Lane 1 : HEK293 cells transfected with VS-REP1
    Lane 2 : HEK293 cells transfected with AtT20

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal anti goat IgG (HRP) at 1/10000 dilution

    Predicted band size: 72 kDa

References

ab192454 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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