Overview

  • Product nameAnti-CHMP2A antibody
    See all CHMP2A primary antibodies
  • Description
    Mouse polyclonal to CHMP2A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-222 of Human CHMP2A

  • Positive control
    • Transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
    • Sequence similaritiesBelongs to the SNF7 family.
    • DomainThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • Cellular localizationLate endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
    • Information by UniProt
    • Database links
    • Alternative names
      • BC2 antibody
      • Charged multivesicular body protein 2a antibody
      • CHM2A_HUMAN antibody
      • CHMP2 antibody
      • CHMP2a antibody
      • Chromatin modifying protein 2a antibody
      • Chromatin-modifying protein 2a antibody
      • hVps2 1 antibody
      • hVps2-1 antibody
      • putative breast adenocarcinoma marker antibody
      • Putative breast adenocarcinoma marker BC-2 antibody
      • Putative breast adenocarcinoma marker BC2 antibody
      • Vacuolar protein sorting associated protein 2 1 antibody
      • Vacuolar protein sorting-associated protein 2-1 antibody
      • Vps2 1 antibody
      • VPS2 antibody
      • Vps2-1 antibody
      • VPS2A antibody
      see all

    Anti-CHMP2A antibody images

    • All lanes : Anti-CHMP2A antibody (ab67064) at 1/500 dilution

      Lane 1 : CHMP2A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 25 kDa
      Observed band size : ~28 kDa (why is the actual band size different from the predicted?)

    References for Anti-CHMP2A antibody (ab67064)

    ab67064 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67064.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"