Overview

  • Product name
  • Description
    Rabbit polyclonal to CHMP2B
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-163 of Human CHMP2B (Q9UQN3).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
  • Tissue specificity
    Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
  • Involvement in disease
    Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
  • Sequence similarities
    Belongs to the SNF7 family.
  • Domain
    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization
    Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS17 antibody
    • Charged multivesicular body protein 2b antibody
    • CHM2B_HUMAN antibody
    • CHMP family, member 2B antibody
    • CHMP2.5 antibody
    • CHMP2b antibody
    • Chromatin modifying protein 2b antibody
    • Chromatin-modifying protein 2b antibody
    • DMT1 antibody
    • hVps2-2 antibody
    • Vacuolar protein sorting 2, yeast, homolog of, B antibody
    • Vacuolar protein sorting 2-2 antibody
    • Vacuolar protein sorting-associated protein 2-2 antibody
    • VPS2 homolog B antibody
    • Vps2-2 antibody
    • VPS2B antibody
    see all

Images

  • Anti-CHMP2B antibody (ab126200) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • ab126200, at 1/500 dilution, staining CHMP2B in methanol-fixed HeLa cells by Immunofluorescence (green). Alpha-tubulin filaments are shown stained red.

References

ab126200 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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