Overview

  • Product name
  • Description
    Rabbit polyclonal to CHMP2B
  • Tested applications
    Suitable for: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human CHMP2B.

    (Peptide available as ab27782.)

  • Positive control
    • Tagged recombinant CHMP2B protein

Properties

Applications

Our Abpromise guarantee covers the use of ab33174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 20420883
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
  • Tissue specificity
    Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
  • Involvement in disease
    Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
  • Sequence similarities
    Belongs to the SNF7 family.
  • Domain
    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization
    Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS17 antibody
    • Charged multivesicular body protein 2b antibody
    • CHM2B_HUMAN antibody
    • CHMP family, member 2B antibody
    • CHMP2.5 antibody
    • CHMP2b antibody
    • Chromatin modifying protein 2b antibody
    • Chromatin-modifying protein 2b antibody
    • DMT1 antibody
    • hVps2-2 antibody
    • Vacuolar protein sorting 2, yeast, homolog of, B antibody
    • Vacuolar protein sorting 2-2 antibody
    • Vacuolar protein sorting-associated protein 2-2 antibody
    • VPS2 homolog B antibody
    • Vps2-2 antibody
    • VPS2B antibody
    see all

Anti-CHMP2B antibody images

  • Anti-CHMP2B antibody (ab33174) at 1 µg/ml + Tagged recombinant CHMP2B protein at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab33174 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33174, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab33174 staining CHMP2B in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. The antibody produced a diffuse and widespread staining in many brain areas. The image shows the staining obtained in the mouse hippocampus.

    See Abreview

  • ab33174 staining CHMP2B in human hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 4% serum for 1 hour at 27°C followed by incubation with the primary antibody at a 1/600 dilution for 18 hours at 4°C. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/500 dilution.

    See Abreview

References for Anti-CHMP2B antibody (ab33174)

This product has been referenced in:
  • Ito M  et al. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology 36:441-447 (2016). Read more (PubMed: 26935872) »
  • Chassefeyre R  et al. Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B. J Neurosci 35:3155-73 (2015). Read more (PubMed: 25698751) »

See all 19 Publications for this product

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse brain, cortex)
Specification
mouse brain, cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 15 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (hippocampus)
Specification
hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Dec 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: distilled water
Permeabilization
No
Blocking step
casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: RT°C
Username

哲也 高橋

Verified customer

Submitted Jul 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up